Formulation and characterization of gellan–DEAE-dextran polyelectrolyte complex hydrogels

Article Sidebar

Published: Dec 17, 2023
DOI: https://doi.org/10.60787/wapcp-27-1-106
Keywords:
hydrogels, polyelectrolyte complexation, binary, DEAE-Dextran, gellan

Main Article Content

Adeola T. Kola-Mustapha
Faculty of Pharmaceutical Sciences University of Ilorin, Ilorin, Nigeria
Amos O. Abioye
 Leicester School of Pharmacy, De Montfort University, Leicester, UK.

Abstract

Background: There is a need to formulate hydrogels via direct physical interaction to circumvent the use of crosslinker which can affect the integrity of the entrapped drug in the hydrogel.


Objective: The aim of this study is to formulate and characterise plain polyelectrolyte complex (PEC) hydrogels prepared by physical interaction between gellan gum and oppositely charged DEAE-Dextran.


Method: Gellan-DEAE-Dextran (GDD) PEC hydrogels were prepared by polyelectrolyte complexation of gellan and DEAE-Dextran. The GDD-PEC hydrogels were characterized using Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA). Their swelling kinetics and rheological properties were also evaluated.


Results: Morphological characteristics analysed by SEM of plain gellan (G) hydrogel showed strand-like smooth compact structured surface while the GDD-PEC hydrogels exhibited crinkled rough surfaces. The shifts and disappearances in peaks shown in the FTIR spectra, suggested an interaction between gellan and DEAEDextran. DSC showed that the gellan and DEAE-Dextran merged into a single endothermic melting peak between 99.8 and 115.3 °C in the GDD-PEC hydrogels. Their TGA showed that the plain gellan (G) hydrogel was able to withstand more heat when compared to the GDD-PEC hydrogels. The GDD-PEC hydrogels exhibited pseudoplastic and elastic characteristics based on the concentration of DEAE-Dextran. The addition of DEAEDextran to GDD-PEC hydrogels significantly increased (p < 0.05, n = 4) their swelling ratio when compared to the plain gellan (G) hydrogel.


Conclusion: GDD-PEC hydrogels were successfully formulated and characterised. This formulation has the potential for use in transdermal drug delivery systems.

Downloads

Download data is not yet available.

Article Details

How to Cite
Kola-Mustapha, A. T., & Abioye, A. O. (2023). Formulation and characterization of gellan–DEAE-dextran polyelectrolyte complex hydrogels. West African Journal of Pharmacy, 27(1), 104-117. https://doi.org/10.60787/wapcp-27-1-106
Issue
Vol. 27 No. 1 (2016)
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Kola-Mustapha, A. T., & Abioye, A. O. (2023). Formulation and characterization of gellan–DEAE-dextran polyelectrolyte complex hydrogels. West African Journal of Pharmacy, 27(1), 104-117. https://doi.org/10.60787/wapcp-27-1-106

Share

References

Peppas NA, Mikos AG (1986). Preparation methods and structure of hydrogels. In: Hydrogels in Medicine and Pharmacy, Peppas NA ed. CRC Press: Boca Raton, Florida: 1-25.

Brannon-Peppas L (1990). Preparation and characterization of crosslinked hydrophilic networks, in absorbent polymer technology,

Brannon-Peppas L, Harland RS, eds. Elsevier: Amsterdam: 45-66.

Peppas NA (1991). Physiologically responsive gels. Jounal of Bioactive and Compatible Polymers 6:241-246.

Park K, Park H (1999) Smart hydrogels. In: Concise Polymeric Materials Encyclopedia. Salamone JC, ed. CRC Press: Boca Raton: 1476-1478.

Qiu Y, Park K (2001). Environment-sensitive hydrogels for drug delivery. Advanced Drug Delivery Reviews 53: 321-339.

Hennink JD, Van Nostrum CF (2002). Novel crosslinking methods to design hydrogels. Advanced Drug Delivery Reviews 54: 13-36.

Peppas NAB, Bures P, Leobandung W, Ichikawa H (2000). Hydrogels in pharmaceutical formulations. European Journal of Pharmaceutics and Biopharmaceutics 50: 27-46.

Saleem MAA, Ali SK, Patil CC (2010). Studies on different chitosan polyelectrolyte complex hydrogels for modified release of diltiazem

hydrochloride. International Journal of Pharmacy and Pharmaceutical Sciences 2(4): 64-67.

Abioye AO, Isa S, Kola-Mustapha AT (2015). Ex vivo skin permeation and retention studies on chitosanibuprofen-gellan ternary nanogel prepared by insitu ionic gelation technique – a tool for controlled transdermal delivery of ibuprofen. International Journal of Pharmaceutics 490 (1-2): 112-130.

Abioye AO, Kola-Mustapha AT, Ruparelia K (2014). Impact of insitu granulation and temperature quenching on crystal habit and micromeritic properties of ibuprofen–cationic dextran conjugate crystanules. International Journal of Pharmaceutics 462 (1-2): 83-102.

Abioye AO, Kola-Mustapha AT, Chi GT, Sunday I (2014). Quantification of in situ granulationinduced changes in pre-compression, solubility,

dose distribution and intrinsic in vitro release characteristics of ibuprofen-cationic dextran conjugate crystanules. International Journal of

Pharmaceutics 471: 453-477.

Abioye AO, Kola-Mustapha AT (2015). Formulation studies on Ibuprofen sodium-cationic dextran conjugate: Effect on tabletting and dissolution characteristics of ibuprofen. Drug Development and Industrial Pharmacy 490 (1-2): 112-130.

O'Neil MA, Selvedran RR, Morris VJ (1983). Structure of the acidic extracellular gelling polysaccharide produced by Pseudonomas

elodea. Carbohydrate Research 124: 123-133.

Rodriguez-Hernadez AID, Garnier S, Tecante CA, Doublier JL (2003). Rheology-structure properties of gellan systems:Evidence of network formation at low gellan concentration. Food Hydrocolloids 17: 621-628.

Yuguchi, Y, Urakawa H, Kajiwara K (1997). Structural characteristics of crosslinking domain in gellan gum gel. Macromolecular Symposia 120:77-89.

Dai LL, Liu XX, Tong YL (2008). Concentration dependence of critical exponents for gelation in gellan gum aqueous solutions upon cooling.

European Polymer Journal 44(12): 4012-4019.

Miyoshi E, Takaya T, Nishinari K (1996). Rheological and thermal studies of gel-sol transition in gellan gum aqueous solutions. Carbohydrate Polymers 30: 109-119.

Sudhamani SR, Prasad MS, Udaya SK (2003). DSC and FTIR studies on gellan and polyvinyl alcohol (PVA) blend films. Food Hydrocolloids 17: 245-250.

Silva-Correia JO, Caridade JM, Oliveira SG, Sousa JT, Mano RA, Reis JF (2011). Gellan gum-based hydrogels for intervertebral disc tissueengineering applications. Journal of Tissue Engineering and Regenerative Medicine 5: e97-e107.

Xu LL, Kennedy B, Xie JF, Huang BJ (2007). Characterization of konjac glucomannam-gellan gum blend films and their sutaibility for release of nisin incorporated therein. Carbohydrate Polymers 70: 192-197.

Pai VS, Khan M (2002). Evolution of microstructure and rheology in mixed polysaccharide systems. Macromolecules 35(5): 1699-1707.

Chenite AB, Wang M, Chaput D, Kandani C (2001). Rheological characterization of thermogelling chitosan/glycerol-phosphate solutions.

Carbohydrate Polymers 46: 39-47.

Moura MJ, Figueiredo MM, Gil MH (2007). Rheological study of genipin cross-linked chitosan hydrogels. Biomacromolecules 8: 3823-3829.

Ferry JD (1980). Viscoelastic properties of polymer. New York: Wiley & Sons: 641.

Montembault A, Viton C, Domard A (2005). Rheometric study of the gelation of chitosan in a hydroalcoholic medium. Biomaterials 26: 1633-1643.

Madsen F, Eberth K, Smart JD (1999). A rheological examination of mucoadhesive/mucus interaction: The effect of mucoadhesive type and

concentration. Journal of Controlled Release 50:167-178.

Nishinari K (1997). Rheological and DSC study of sol-gel transition in aqueous dispersions of industrially important polymers and colloids.

Colloid Polymer Science 275: 1093-1107.