Hepato-protective activity of ethanol extract of Moringa oleifera leaves in acute rifampicin-induced hepatotoxicity Activité hépatoprotectrice de l'extrait éthanolique de feuilles de <i>Moringa oleifera</i> dans l'hépatotoxicité aiguë induite par la rifampicine
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Abstract
ENGLISH
Background: Drugs are an important cause of liver injury. The use of natural remedies for the treatment of liver diseases has a long history, and medicinal plants are still used all over the world for this purpose. For their medicinal and nutritional values, the Moringa oleifera plant is of particular interest.
Objective: In the current study, we aimed to explore the hepato-protective effects of ethanol extract of Moringa oleifera leaves in acute rifampicin-induced hepatotoxicity.
Methods: Thirty mice (18-20 g) were randomly assigned into 6 groups (n = 5). Group I received normal saline only; groups II, III, IV, V, and VI were administered with single oral rifampicin 400 mg/kg, treated with normal saline, different doses of ethanol extract of Moringa oleifera (200, 400, and 800 mg/kg), and ascorbic acid, respectively. Serum levels of liver enzymes Alanine transaminase (ALT), Aspartate transaminase (AST), Alkaline phosphatase (ALP), catalase and superoxide dismutase (SOD) were measured in the serum. Furthermore, phytochemical constituents were also measured in the Moringa oleifera leaves extract.
Results: Treatment with an ethanol extract of Moringa oleifera decreased liver enzyme levels and improved oxidative stress status in hepatotoxic mice in a dose-dependent manner.
Conclusion: Normal levels of liver enzymes, an increase in total antioxidant capacity, and a decrease in lipid peroxidation level uncovered the hepatoprotective effects of the ethanol extract of Moringa oleifera. It seems the antioxidant and hepatoprotective effects of botanical extracts are basically linked with their phenol and flavonoid properties that neutralize oxidant agents. However, more studies are required to implement our strategy.
FRENCH
Contexte: Les médicaments sont une cause importante de lésions hépatiques. L'utilisation de remèdes naturels pour le traitement des maladies du foie a une longue histoire et les plantes médicinales sont encore utilisées à cette fin dans le monde entier. Pour ses valeurs médicinales et nutritionnelles, la plante Moringa oleifera présente un intérêt particulier.
Objectif: Dans la présente étude, nous avons cherché à explorer les effets hépatoprotecteurs de l'extrait éthanolique de feuilles de Moringa oleifera dans l'hépatotoxicité aiguë induite par la rifampicine.
Méthodes: Trente souris (18-20 g) ont été réparties au hasard en 6 groupes (n = 5). Le groupe I a reçu uniquement une solution saline normale ; les groupes II, III, IV, V et VI ont reçu une seule dose orale de rifampicine de 400 mg/kg, traités avec une solution saline normale, différentes doses d'extrait éthanolique de Moringa oleifera (200, 400 et 800 mg/kg) et de l'acide ascorbique, respectivement. Les taux sériques des enzymes hépatiques alanine transaminase, aspartate transaminase, lactate déshydrogénase et phosphatase alcaline, glutathion réduit (GSH), catalase, superoxyde dismutase et peroxydation lipidique sont mesurés dans le tissu hépatique à l'aide de kits spécifiques à la fin de la période expérimentale. En outre, les constituants phytochimiques ont également été mesurés dans l'extrait de feuille de Moringa oleifera.
Résultats: Le traitement avec un extrait éthanolique de Moringa oleifera a diminué les niveaux d'enzymes hépatiques et amélioré l'état de stress oxydatif chez des souris hépatotoxiques de manière dose-dépendante.
Conclusion: Des niveaux normaux d'enzymes hépatiques, une augmentation de la capacité antioxydante totale et une diminution du niveau de peroxydation lipidique ont mis en évidence les effets hépatoprotecteurs de l'extrait éthanolique de Moringa oleifera. Il semble que les effets antioxydants et hépatoprotecteurs des extraits botaniques soient essentiellement liés à leurs propriétés phénoliques et flavonoïdes qui neutralisent les agents oxydants. Cependant, des études supplémentaires sont nécessaires pour mettre en œuvre notre stratégie.
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