Phytochemical, antioxidant and antimicrobial studies of partitioned fractions of Lannea kerstingii Engl. and K. Krause (Anarcadiaceae)
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Abstract
Background: Antimicrobial resistance is a serious threat to global public health and requires actions like the development of new antimicrobial with significant activities over existing drugs.
Objectives: This study aims at investigating the phytochemical, antioxidant and antimicrobial potentials of partitioned fractions of Lannea kerstingii.
Methods: A quantitative phytochemistry of chloroform, ethyl acetate, acetone and methanol fractions of L. kerstingii for total flavonoid, phenol and alkaloid were done using standard method; antioxidant and antimicrobial activity were determined using 1,1-Diphenyl-2-picrylhydrazyl and agar diffusion method respectively.
Results: The chloroform fraction contained only alkaloid (11%) and steroids while all the other fractions contained phenolic compounds in the range 0.43 to 0.67 mg/g garlic acid. The total flavonoid content ranged from 0.43 to 0.67 mg/g of quercetin. The total flavonoid in the ethyl acetate was significantly different from that of the methanol fraction but not with the acetone fraction. The acetone fraction showed highest antioxidant activity (60.4%) at 0.05 mg/mL though not as comparable to vitamin C. The ethyl acetate showed high antimicrobial activity as it was active against most of the organisms tested upon and zone of inhibition ranged from 13±0.02 to 29±0.1 mm. The acetone fraction was active only against T. mentagrophytes while the methanol fraction showed no activity.
Conclusion: The antioxidant and antimicrobial activities may be due to the presence of flavonoids, as well as the presence of tannins and terpenoids present in the different fractions. This makes the ethyl acetate fraction a good source of antioxidant and antimicrobial agent.
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References
Kane JH, Finlay AC and Sobin BA (1950). Antimicrobial agents from natural sources. Annals of the New York Academy of Sciences 53:226-228
Hashemi SH, Esna-Ashari F, Tavakoli S and Mamani M (2013). The prevalence of antibiotic resistance of Enterobacteriaceae strains isolated in communityand hospital-acquired infections in teaching hospitals of Hamadan, west of Iran. Journal of Research in Health Sciences 13(1):75-80
Tawadrous GA, Aziz AA, Amin DG, Eldemery A and Mostafa MAA (2012). RANTES, TNF-alpha, oxidative stress, and hematological abnormalities in hepatitis C virus infection. Journal of Investigative Medicine 60(6):878-882
Kohanski MA, Dwyer DJ, Hayete B, Lawrence C and Collins J (2007) A common mechanism of cellular death induced by bactericidal antibiotics, Cell 130:797-810.
Van Acker H and Coenye T (2017). The role of reactive oxygen species in antibiotic-mediated killing of bacteria, Trends Microbiol. 25:456-466.
Zengin G, Cakmak YS, Guler GO and Aktumsek A (2011). Antioxidant properties of methanolic extract and fatty acid composition of Centaurea urvillei DC. subsp. Hayekiana Wagenitz. Records of Natural Products 5:123-132.
Halliwell B and Gutteridge JMC (1981). Formation of thiobarbituric acid reactive substances from deoxyribose in the presence of iron salts: the role of superoxide and hydroxyl radicals. FEBS Letters 128:347-352.
Gulcin I (2012). Antioxidant activity of food constituents: an overview. Archives of Toxicology 86:345-391.
Nunes PX, Silva SF, Guedes RJ and Almeida S (2012). Biological oxidations and antioxidant activity of natural products, Phytochemicals as nutraceuticals - Global Approaches to Their Role in Nutrition and Health.
Wannes WA, Mhamdi B, Sriti J, Jemia MB, Ouchikh O, Hamdaoui G, Kchouk ME and Marzouk B (2010). Antioxidant activities of the essential oil and methanol extracts from myrtle (Myrtuscommunis var. italica L.) leaf, stem and flower. Food and Chemical Toxicology 48:1362-1370.
Verma S and Singh S (2008). Current and future status of herbal medicines. Veterinary world 1(11):347-350
Oluyemi KA, Okwuonu UC, Baxter DG and Oyesola TO (2007). Toxic effects of methanolic exract of Aspilia africana leaf on the estrous cycle and uterine tissues of Wistar rats. International Journal of Morphology 25:609-614
Doka IG and Yagi SM (2009). Ethnobotanical Survey of Medicinal Plants in West Kordofan (Western Sudan). Ethnobotanical Leaflets.13:1409-1416
Diallo A, Eklu-Gadegkeku K, Mobio T, Moukha S, Agbonon A, Aklikokou K, Creppy EE and Gbeassor M (2009). Protective effect of Moringa oleifera Lam. and Lannea kerstingii extracts against cadmium and ethanol-induced lipid peroxidation. Journal of Pharmacology and Toxicology 4:160-166
Koné WM, Soro D, Dro B, Yao K and Kamanzi K (2011). Chemical Composition, Antioxidant, Antimicrobial and Acetylcholinesterase Inhibitory Properties of Lannea Barteri (Anacardiaceae). Australian Journal of Basic and Applied Sciences 5(10);1516-1523.
Njinga NS, Sule MI, Patteh UU, Hassan HS, Usman MA and Haruna MS (2013). Phytochemical and Antidiarrhea Activity of the Methanolic Extract of the Stem Bark of Lannea kerstingii Engl. and K. Krause (Anacardiaceae). Journal of Natural Product and Plant Resources 3(3):43-47.
Njinga NS, Sule MI, Pateh UU, Hassan HS, Ahmad MM, Abdullahi ST, Danja BA and Bawa B (2014). Phytochemical and antimicrobial activity of the leaves of Lannea kerstingii Engl & K. Krause (anacadiaceae). Nitte University Journal of Health Science 2014;4(4):4-9.
Njinga NS, Sule MI, Pateh UU, Hassan HS, Abdullahi ST and Ache RN (2016). Isolation and Antimicrobial Activity of ?-Sitosterol-3-O-Glucoside from Lannea kerstingii Engl. & K. Krause (Anacardiacea). Nitte University Journal of Health Science. 6(1):4-8.
Harborne JB (1973). Phytochemical Methods London Chapman and Hall, Ltd.49-188.
Sofowora A (1993). Medicinal Plants and Traditional Medicinal in Africa. 2nd Ed. Sunshine House, Ibadan, Nigeria: Spectrum Books Ltd. Screening Plants for Bioactive Agents.134-156.
Trease GE and Evans WC (2002). Pharmacognosy. 15th edition. Saunders Publishers, London.
Adedapo A, Jimoh F, Koduru S, Masika J and Afolayan A (2009). Assessment of the medicinal potentials of the methanol extracts of the leaves and stems of Buddlejasaligna. BMC Complementary and Alternative Medicine 9:9-21
Nabavi SM, Ebrahimzadeh M, Nabavi S, Hamidinia A and Bekhradnia AR (2008). Determination of antioxidant activity, phenol and flavonoids content of Parrotia persica MEY. Pharmacology online 2008;2:560-567.
Kumar T. and Jain V (2015). Appraisal of Total Phenol, Flavonoid Contents, and Antioxidant Potential of Folkloric Lannea coromandelica Using In Vitro and In Vivo Assays. Scientifica. Volume 2015 Article ID203679
Diallo A, Eklu-Gadegbeku K, Agbonon A, Aklikokou K, Napo-Koura G, Creppy E and Gbeassor M (2017). Repeated-dose toxicological studies of hydroalcoholic extract of Lannea kerstingii Engl and K. krause (anacardiaceae) and identification of toxicity mechanisms. International Journal of Pharmaceutical Sciences And Research. 6(2): 604-611
Obadoni BO and Ochuko PO (2001). Phytochemical studies and comparative efficacy of the crude extracts of some homeostatic plants in Edo and Delta State of Nigeria. Global Journal of Pure and Applied Sciences 8:203-208
Ndhlala AR, Finnie JF and Van Staden J (2010). In vitro antioxidant properties, HIV-1 reverse transcriptase and acetylcholinesterase inhibitory effects of traditional herbal preparations sold in South Africa. Molecules. 15:6888-6904.
Lino A and Deogracios O (2006). The in-vitro antibacterial activity of Annona senegalensis, Securidaccalongi pendiculata and Steanotaenia araliaceae, Ugandan Medicinal plants. African Health Sciences 2006;6(1):31-35
Krishnaiah D, Sarbatly R and Nithyanandam RR (2011). A review of the antioxidant potential of medicinal plant species. Food and Bioproducts Processing 89:217-233.
Aberoumand A and Deokule SS (2008). Comparison of phenolic compounds of some edible plants of Iran and India. Pakistan Journal of Nutrition 7:582-585.
Hayouni EA, Abedrabba M, Bouix M and Hamdi M (2007). The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chemistry 105:1126-1134.
Tung YT, Wub HJ, Hsieh C, Ping-Sheng Chen PS and Chang ST (2009). Free radicalscavenging phytochemicals of hot water extracts of Acacia confusa leaves detected by an on-line screening method. Food Chemistry115:1019-1024.
Di Carlo G, Mascolo N, Izzo AA and Capasso F (1999). Flavonoids: old and new aspects of a class of natural therapeutic drugs. Life Sciences 65:337-353.
Montoro P, Braca A, Pizza C and De Tommasi N (2005). Structure-antioxidant activity relationships of flavonoids isolated from different plant species. Food Chemistry 92:349-355.
Deji-Agboola AM (2010). Antimicrobial Activity of Lannea welwitschii Stem Bark Against Wound Pathogens. Ethiopian Pharmaceutical Journal. 28:2 (2010).