Antimicrobial studies of the leaf extract of Argemone mexicana and its ointment formulation
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Abstract
Background: Skin infections are rampant in children and there is a widespread resistance to common antibacterial and antifungal therapies for these infections.
Objective: This study aims to screen the extract of Argemone mexicana leaf for antibacterial and antifungal activity; and formulate the extract into ointment dosage forms using simple ointment B.P.C.
Methods: Preliminary phytochemical studies were performed and the methanol, ethanol and n-hexane leaf extracts of Argemone mexicana Linn. were evaluated for antimicrobial activity against some pathogenic bacteria- Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia(Gram negative) and Streptococcus spp, Staphylococcus aureus (Gram positive) and dermatophytes such as Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum audouinii (fungi) using well diffusion method. The most active extract was formulated into ointment and evaluated for physicochemical and antimicrobial activity.
Results: Flavonoids and alkaloids were present in all the extracts while anthraquinones and saponin were present in methanol and n hexane extract. The ranking for extract activity was methanol>n- hexane> ethanol. The methanol extract showed comparable activity with gentamicin against Staphylococcus aureus and higher activity against Escherichia coli at 250mg/ml and 500mg/ml. The order of activity of the methanol extract against the test organisms is Staphylococcus aureus> Escherichia coli> Streptococcus spp> Klebsiella pneumonia> Trichophyton mentagrophyte> Trichophyton rubrums> Microsporum audouinii. There was no significant variation (P> 0.05) in the zones of inhibition of bacteria with the methanol extract. The formulated ointment had a characteristic odour, smooth texture, was easy to apply on rubbing, easy to remove with soap and water and non-irritant on skin.
Conclusion: The result of this study supports the traditional uses of these plants and shows that the plant extract possesses antibacterial and antifungal activities
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References
Sarma DR and Toshniwal SK (1963). Pattern of skin diseases in Indore. Indian Journal of Paediatrics 30(10): 335-338.
Sladden MJ and Graham AJ (2004). Common skin infections in children. British Medical Journal 329: 95
Stulberg DL, Penrod MA and Blatny RA (2002). Common bacterial skin infections. American Family Physician 66(1): 119–24.
Siddiqui IA, Shaukat SS, Khan GH and Zaki MJ (2002). Evaluation of Argemone mexicana for control of root- infecting fungi in potato. Journal of Phytopathology 150(6): 321-329.
Trease GE and Evans WC (2002): Pharmacognosy, 15th Edition. Saunders, London, pp. 42-44
Harborne JB (1998). Phytochemical methods: A guide to modern techniques of plant analysis, 3rd Edition. Chapman & Hall Pub. London,
UK, pp. 221-229.
Odebiyi OO and Sowofora EE (1978). Phytochemical Screening of Nigeria Medicinal Plants. Lioydia 41: 234-246
Sofowora A (1993). Screening plants for bioactive agents. In: Sofowora (Eds). Medicinal Plants and Traditional Medicine in Africa. Spectrum Books Ltd, Nigeria, pp. 134–156.
Adeniyi BA, Odelola HA and Oso BA (1996). Antimicrobial potentials of Diospyros mespiliformis (Ebenaceae). African Journal of Medical Sciences 25(3): 221–224.
Esimone CO, Adikwe MU and Okonta JM (1998). Preliminary antimicrobial screening of the ethanolic extract from lichen Usnea
Subfloridans (L) Journal o f Pharmaceutical Research and Development 3(2): 99-101.
Doughari JH, Elmahmood AM and Manzara S (1997). Studies on the antibacterial activity of the root extracts of Carica papaya. African Journal of Microbiological Research 1(3): 37-41.
Lima ED (1994). Chemical composition and antimicrobial activity of essential oil from Brazilian plant, Fitoterapia 63: 371-37.
Akinpelu DA and Soetan MA (2006). Antimicrobial activities of medicinal folklore remedies in south western Nigeria. African Journal of
Biotechnology 5(11): 1078-1081.