Effect of concomitant administration of sildenafil citrate and selected herbal bitters on sex hormones and some biochemical indices in Wistar rats Effet de l'administration concomitante de citrate de sildénafil et de certaines plantes amères (bitters) sur les hormones sexuelles et certains paramètres biochimiques chez le rat Wistar
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Abstract
Background: The increase in prevalence of male sexual dysfunction particularly erectile dysfunction has resulted in males consuming various types of products to improve their sexual life.
Objective: This study was designed to determine the effect of simultaneous administration of sildenafil citrate and herbal bitters on LH and testosterone, and a few biochemical markers (urea, creatinine, ALP, AST, and ALT) in Wistar rats.
Methods: Forty (40) male Wistar rats with weight range of 115 g to 125 g were divided into 8 groups. Group A was orally given distilled water and rats' feed as control, Group B was orally administered water, rats' feed and sildenafil citrate, Group C was orally administered water, rats' feed and orijin bitters, Group D was orally administered water, rats' feed and Action bitters, Group E was orally administered water, rats' feed and Coco Samba bitters, Group F was orally administered water, rats' feed and sildenafil citrate plus orijin bitters, Group G was orally administered water, rats' feed and sildenafil citrate plus action bitters and Group H was orally administered water, rats' feed and sildenafil citrate and Coco Samba bitters for 42 days. Blood samples (5 mL each) were collected from various rats and used for biochemical analysis.
Results: A statistically significant increase in AST, ALT, Urea, LH and testosterone was seen across all experimental groups in comparison with the control group (p < 0.05). The result also showed a statistically significant increase in creatinine in coco samba bitters administered Wistar rats and rats given coco samba butters concurrently with sildenafil citrate (p < 0.05).
Conclusion: Results obtained from this study revealed significant benefit of concomitant administration of herbal bitters and sildenafil citrate, causing an increase in LH and testosterone which can have a positive impact in men experiencing sexual disorders like erectile dysfunction. There is also potential deleterious effect of concomitant administration of sildenafil citrate and herbal bitter on male Wistar rats with elevation of serum level of urea, creatinine, AST, and ALT.
Résumé
Contexte: L'augmentation de la prévalence des dysfonctions sexuelles masculines, en particulier des troubles de l'érection, a conduit les hommes à consommer divers types de produits pour améliorer leur vie sexuelle.
Objectif: Cette étude a été conçue pour déterminer l'effet de l'administration simultanée de citrate de sildénafil et d'herbes amères sur l'hormone lutéinisante (LH) et la testostérone, ainsi que sur quelques paramètres biochimiques (urée, créatinine, ALP, AST et ALT) chez les rats Wistar.
Méthodes: Quarante (40) rats mâles Wistar, pesant entre 115 g et 125 g, ont été répartis en 8 groupes. Le groupe A a reçu par voie orale de l'eau distillée et de la nourriture pour rats (groupe témoin). Le groupe B a reçu par voie orale de l'eau, de la nourriture pour rats et du citrate de sildénafil. Le groupe C a reçu par voie orale de l'eau, de l'aliment pour rats et Orijin bitters. Le groupe D a reçu par voie orale de l'eau, de la nourriture pour rats et Action bitters. Le groupe E a reçu par voie orale de l'eau, de la nourriture pour rats et Coco Samba bitters. Le groupe F a reçu par voie orale de l'eau, de la nourriture pour rats, du citrate de sildénafil et Orijin bitters. Le groupe G a reçu par voie orale de l'eau, de la nourriture pour rats, du citrate de sildénafil et Action bitters. Enfin, le groupe H a reçu par voie orale de l'eau, de la nourriture pour rats, du citrate de sildénafil et Coco Samba bitters. L'expérience a duré 42 jours. Des échantillons de sang (5 mL chacun) ont été prélevés chez différents rats et utilisés pour des analyses biochimiques.
Résultats: Une augmentation statistiquement significative des taux d'AST, d'ALT, d'urée, de LH et de testostérone a été observée dans tous les groupes expérimentaux par rapport au groupe témoin (p < 0.05). Les résultats ont également montré une augmentation statistiquement significative de la créatinine chez les rats Wistar ayant reçu Coco Samba bitters et chez les rats ayant reçu simultanément Coco Samba bitters et du citrate de sildénafil (p < 0.05).
Conclusion: Les résultats de cette étude révèlent un bénéfice notable de l'administration concomitante de bitters à base de plantes et de citrate de sildénafil, entraînant une augmentation de LH et de testostérone, ce qui peut avoir un effet positif chez les hommes souffrant de troubles sexuels tels que la dysfonction érectile. Cependant, cette administration concomitante pourrait avoir un effet délétère chez le rat Wistar mâle, se traduisant par une élévation des taux sériques d'urée, de créatinine, d'AST et d'ALT.
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