Investigation of herb-drug interaction between MAMA powder herbal antimalarial remedy and amodiaquine, chloroquine, artesunate in murine malaria Étude de l'interaction plante-médicament entre le remède antipaludique à base de plantes en <i>poudre MAMA</i> et l'amodiaquine, la chloroquine et l'artésunate dans le cas du paludisme murin
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Abstract
ENGLISH
Background: Antimalarial herbal remedies are traditionally presented as infusions, decoctions and extracts. There is limited information on antimalarial herbal powder presentations. The outcomes of possible co-administration of MAMA POWDER (MP), a herbal powder listed for malaria treatment, with orthodox antimalarial drugs, had not been explored.
Objectives: This study investigated MP as a reduced particle-sized powder, and its effects on amodiaquine (AQ), chloroquine (CQ), artesunate (AS) in mice.
Methods: MAMA POWDER comprises the powdered stem bark of Alstonia boonei De Wild (Apocynaceae) and seed of Picralima nitida H Durand (Apocynaceae). The barks were milled, sieved (particle size, 180 μm), mixed and suspended in propylene glycol (50 %). This suspension was assessed on chloroquine-sensitive and chloroquine-resistant Plasmodium berghei strains in varying oral doses (0.39-100 mg/kg), using the chemosuppressive and curative antimalarial test models. Co-administration of MP with the orthodox drugs was similarly investigated as follows: (MP 1.56+AQ 10; MP 6.25+AQ 10), (MP 1.56+CQ 10; MP 6.25+CQ 10), (MP 1.56+AS 4; MP 6.25+AS 4)
mg/kg.
Results: MP suspension (6.25 mg/kg) exhibited considerable antimalarial chemosuppression (82 %) and it enhanced the activity of AQ (MP 6.25+AQ 10 mg/kg) in chloroquine-sensitive malaria infection (91 %). Significant antagonistic interactions were observed with the combinations of MP (1.56 mg/kg, 69%) and CQ (55.2 %) respectively.
Conclusion: MP exhibited significant antimalarial activity. The potentiation of AQ and antagonism with CQ, suggested herb-drug interactions, which called for further investigation.
FRENCH
Contexte: Les remèdes antipaludiques à base de plantes se présentent traditionnellement sous forme d'infusions, de décoctions et d'extraits. Les informations sur les présentations de poudres antipaludiques à base de plantes sont limitées. Les effets d'une éventuelle co-administration de MAMA POWDER (MP), une poudre à base de plantes indiquée pour le traitement du paludisme, avec médicaments antipaludiques orthodoxes n'ont pas été étudiés.
Objectifs: Par conséquent, cette étude a examiné le MP sous forme de poudre de taille de particules réduite et ses effets sur l'amodiaquine (AQ), la chloroquine (CQ) et l'artésunate (AS) chez la souris.
Méthodes: MAMA POWDER comprend l'écorce de tige en poudre d'Alstonia boonei De Wild ( Apocynacées) et la graine de Picralima nitida H Durand (Apocynacées). Les écorces ont été broyées, tamisées (granulométrie : 180 µm), mélangées et mises en suspension dans du propylène glycol (50%). Cette suspension a été évaluée sur des souches de Plasmodium berghei sensibles et résistantes à la chloroquine à différentes doses orales (0,39-100mg/kg), en utilisant les modèles de test antipaludique chimiosuppresseur et curatif. La co-administration de MP avec les médicaments orthodoxes a également été étudiée de la manière suivante : (MP 1,56 + AQ 10 ; MP 6,25 + AQ 10), (MP 1,56 + CQ 10 ; MP 6,25 + CQ 10), (MP 1,56 + AS 4 ; MP 6,25 + AS 4) mg/kg.
Résultats: La suspension de MP (6,25 mg/kg) a montré une chimio-suppression antipaludique considérable (82%) et a amélioré l'activité de l'AQ (MP 6,25 + AQ 10 mg/kg) dans l'infection paludique sensible à la chloroquine (91%). Des interactions antagonistes significatives ont été observées avec les combinaisons de MP (1,56 mg/kg, 69 %) et de CQ (55,2 %) respectivement.
Conclusion: Le MP a montré une activité antipaludique significative. La potentialisation de l'AQ et l'antagonisme avec la CQ ont suggéré des interactions entre les plantes et les médicaments, ce qui nécessite des recherches plus approfondies.
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