Antibiotic susceptibility profile of isolates from pregnant women with asymptomatic bacteriuria attending antenatal clinics in three hospitals in Kano, Nigeria
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Abstract
Background: Pregnancy enhances the progression from asymptomatic to symptomatic bacteriuria which could lead to pyelonephritis and adverse obstetric outcomes. This study is aimed at determining the prevalence of asymptomatic bacteriuria and antibiotic susceptibility testing of uropathogens from pregnant women.
Objectives: This experimental study was done to determine the antibiotic susceptibility profile of uropathogens from antenatal patients.
Method: Three hundred and ten asymptomatic women attending antenatal clinics were sampled for the presence of bacteria. Antibiotic susceptibility test of isolates were carried out using Kirby-Bauer disc diffusion method.
Result: The prevalence of asymptomatic bacteriuria was found to be 15.2%, and Proteus spp (49.0%) the most prevalent organism. Antimicrobial susceptibility profile of Proteus spp. and E. coli to some commonly prescribed antibiotics showed resistance to amoxicillin, ciprofloxacin, ceftriaxone, nitrofurantoin, gentamicin, levofloxacin, cotrimoxazole, amoxicillin/clavulanic acid, nalidixic acid and tetracycline (47- 100%). S. aureus showed resistance to ceftriaxone, nitrofurantoin, amoxicillin/clavulanic acid, nalidixic acid and tetracycline (66.7-100%). The multiple antibiotic resistance index (MARI) observed in this study with reference to the tested antibiotics showed that 99.96% of the Proteus spp. isolates have MAR Index of 0.2 to 1.0, while 99.97% of the E. coli isolates have MAR Index of 0.2 to 1.0.
Conclusion: These call for rational use of antibiotics in antenatal clinics to limit the emergence and transmission of multiple antibiotic resistant strains, more so when there is a change of pattern in the prevailing organism in asymptomatic bacteriuria which must be treated in pregnancy in order to prevent adverse maternal and neonatal complications.
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References
Imade PE, lzekor PE, Eghafona NO, Enabulele I, Ophori, E (2010). Asymptomatic bacteriuria among pregnant women. North American Journal of Medical Science, 2(6): 263-266.
Gilbert DN, Moelleving Jr RC, Eliopoulos GN, Sander NA (2005). Sandford guide to Antimicrobial therapy. 32 Hyde Park, Vermont: Antimicrobial Therapy, Inc. pp 22-23.
Connolly A, Thorp JM Jnr (1999). Urinary tract infection in pregnancy. Urology Clinical North America 26(4): 779-787.
Nicolle LE (1994). Screening for asymptomatic bacteriuria in pregnancy. Canadian guide on preventive healthcare, Ottawa Health, Canada; pp 100-106.
Delzell JE Jr, and Leferre, ML (2000). Urinary tract infections during pregnancy. American Family Physician 61(3): 713-721.
Zhao J and Wu LF (2004). Study of the causes of fetal growth restriction with unclear etiologies. Zhonghau Fu Chan Ke Za Zhi, 39: 329-33.
Ryan KJ, Berkowitz R, and Barbieri RL (1990). Kistner's Gynaecology: Principle and Practice. 5 Edn. Appleton. Boston, pp 300-302.
Hill JB, Sheffield JS, Mc Intire DD, and Wendel GD (2005). Acute pyelonephritis in pregnancy.Obstetrics and Gynaecology. 105: 18-23.
Guyton CA (1996) Medical physiology. 9 Edn. W.B. Saunders Company. Philadelphia, pp: 372-373.
Lindsay EN (2003). Screening for asymptomatic bacteriuria in pregnancy. Canadian Guide to Clinical preventive Health care, 1: 100-106.
Bloomberg B, Olsen BE, Hinderaker SG, Langeland N, and Gasheka P (2005). Antimicrobial resistance in urinary bacterial isolates from pregnant women in rural Tanzania: Implications for public health. Scandinavian Journal of Infectious Diseases, 37:262-268.
Ogbolu DO, Akinloye O, Terry Alli OA, Akinloye OM (2006). Asymptomatic bacteriuria of pregnancy in Ibadan, Nigeria: a re-assessment. British Journal of biomedical science; 63(3): 109-112.
Amadi ES, Enemuo OB, Uneke CJ, Nwosu OK, Onyeagba RA, and Ugbogu OC (2007). Asymptomatic bacteriuria among pregnant women
in Abakaliki, Ebonyi State, Nigeria. Journal of Medical Science. 7(4): 698-700.
Akerele J, Abhulimen P, and Okonofua (2001). Prevalence of asymptomatic bacteriuria, among pregnant women in Benin City, Nigeria. Journal of Obstetrics and Gynaecolology, 21: 141-144.
Paul S, Bezbarauh RL, Roy, MK, and Ghosh, AC (1997). Multiple antibiotic resistance (MAR) Index and its reversion in Pseudomonas aeruginosa. Letters in Apllied Microbiology. 24: 169-171.
Hooton TM, Scholees D, Stapleton AF (2000). A prospective study of asymptomatic bacteriuria in sexually active young women. New Eng1and Journal of Medicine; 343:992-7.
Cheesbrough M (2002). District Laboratory practice in Tropical countries. Part 2. Cambridge University press, U.K., pp. 391.
Cowan ST (1974). Cowan and Steel's manual for the identification of Medical bacteria (2nd edition). Cambridge University Press, Cambridge, pp. 110-118.
Collee J, and Miles RS (1989). Tests for identification of bacteria. In: College JR, Duguid JP, Fraser AG, Marmion BP (eds). Mackie and McCartney's Practical Medical Microbiology. 13 ed. Churchill Livingstone/Longman, London. Pp.141-160.
Bauer AW, Kirby WM, Sherris JC, Turch M (1996). Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology. 45:493-497.
Wolf PL (1975). Practical Clinical Microbiology. Techniques and interpretations. John Wiley and Sons. Inc. New York. pp 186-188.
Clinical Laboratory Standards Institute (2006). M 1 0 0 - S 1 6 . Performance Standards for Antimicrobial Susceptibility Testing. Sixteenth
Informational Supplement. 26 (3).
Olusanya O, Ogunledum A, Fakoya TA (1993).Asymptomatic bacteriuria among pregnant and non-pregnant women in Sagamu, Nigeria. West African Journal of Medicine. 12(1): 27-3
Durowaiye MT, Onaolapo JA, Oyi AR (2011). Preliminary study on asymptomatic bacteriuria in pregnant women attending antenatal clinics in three major hospitals, Kano, Nigeria. Nigerian Journal of Pharmaceutical Sciences. 10(2):15-21.
Krumperman PH (1983). Multiple Antibiotic Indexing Escherichia coli to identifying risk sources of feacal contamination of foods. Applied and Environmental Microbiology. 46: 165-170.