Antimicrobial activities of lactic acid bacteria against Pseudomonas aeruginosa, Providencia vermicola, Alcaligenes faecalis and methicillin resistant S. aureus
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Abstract
Background: There is rapid emergence of drug-resistant methicillin resistant S. aureus (MRSA) pseudomonads and enterobacteriaceae strains, however little information are available on effects of lactic acid bacteria (LAB) on some enterobacteriaceae strains.
Objective: This study was to assess the antimicrobial effect of some lactobacilli and Weisella strains against selected potential pathogens (Pseudomonas aeruginosa, MRSA, Providencia vermicola and Alcaligenes faecalis) in co-culture.
Methods: The antibiotic susceptibility of the selected potential pathogens was done by disc diffusion method and cell free supernatant of fourteen different strains of LAB was initially tested against the selected potential pathogens by agar diffusion method..The antimicrobial effects of Lactobacillus plantarum 9, Lactobacillus buchneri SM04, Lactobacillus fermentum 008, Lactobacillus brevis 21, and Weissella paramesenteroides BS03 were tested against the four selected potential pathogens by two different co-culture methods at different contact times.
Results: The tested potential pathogens were generally resistant to tested antibiotics with Providencia. vermicola exhibiting 100% resistance. Lactobacillus brevis 21, Lactobacillus buchneri SM04, Lactobacillus plantarum 9, Lactobacillus fermentum 008, and Weissella paramesenteroides BS03 have antimicrobial activities against the tested potential pathogens in cell free supernatant experiment. The five tested LAB inhibited the potential pathogens in co-culture but at different contact time. Lactobacillus fermentum 008 totally inhibited the growth of Providencia vermicola after 18 hours of co-culture while all the potential pathogens were inhibited in overnight culture of LAB.
Conclusion: Lactic acid bacteria have great antagonistic activities against the tested potential pathogens used in this study. They therefore have great potentials as alternative therapy in cases where antibiotics resistance has been established.
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