Isolation of an Antibacterial Principle from Terminalia ivorensis chev. Stem bark
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Abstract
Background: Widespread resistance to current antibiotic therapies has necessitated the search for new antiinfectives from natural and synthetic sources. Ethnobotanical information indicated the use of Terminalia ivorensis in the treatment of wounds, syphilis and inflammation.
Objectives: This study was undertaken to investigate the antibacterial properties of the Terminalia ivorensis extract and isolate the bioactive compound from the extract.
Methods: Sensitivity of four nosocomial microorganisms (Bacillus substilis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus) to the acetone extract of T. Ivorensis was investigated by agar cup diffusion method. Bioguided solvent–solvent fractionation was carried out on the extract. The most active fraction was subjected to open column chromatography to obtain a bioactive compound. The compound was characterized by application of spectroscopic techniques (ESI-MS, HR-MS, 1D and 2D NMR).
Results: Four microorganisms were sensitive to the whole extract. Column separation led to the isolation of 2- (3,5-dihydroxyphenyl)-benzofuran-5,6-diol as an active principle in the extract. MIC values obtained for the bioactive compound and the whole acetone extract were 1.25mg/ml and 12.5mg/ml respectively.
Conclusion: This study suggests that there is a scientific basis for the application of T. ivorensis extract in the treatment of infections. Further studies on its in vivo activities and the toxicity profile are required before application in humans.
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References
Memmune S, Hilal Y, Neva G, Bulent C, Zeynep E and Sezai E (2009). Total phenolic content, antioxidant and antimicrobial activities of some
medicinal plants; Pak. J. Pharm. Sci 22(1):102-106.
Samuel BB, Okogun JI, Idowu K and Olaniyi AA (2009). Phytochemical and antisickling activities of Terminalia catappa (Combretaceae) leaf extracts. Journal of Phytomedicine and Therapeutics 14:31-36.
Yang SF, Chen MK, Hsieh YS, Yang JS, Su SC, Kao TY, Chen PN and Chu SC(2010). Antimetastatic effects of Terminalia catappa L. on oral cancer via a down regulation of metastasis-associated proteases. Food Chem Toxicol 48(4):1052-8.
Fyhrquist P, Mwasumbi L, Vuorela H and Vuorela H (2006). Preliminary antiproliferative effects of some species of Terminalia, Combretum and Pteleopsis collected in Tanzania on some human cancer cell lines. Fitoterapia 77(5):358–66
Anam K, Widharna RM and Kusrini D (2009). glucosidase inhibitor activity of Terminalia species. Int. J. Pharmacol 5(4):277–80.
Bag A, Bhattacharyya SK and Chattopadhyay RR (2013). Therapeutic potential of Terminalia chebula Retz (Combretaceae): The Ayurvedic
wonder 3(3):244–52.
Adewunmi CO, Agbedahunsi JM, Adebajo AC, Aladesanmi N Murphy and Wando J (2001). Ethnoveterinary medicine: screening of Nigerian
medicinal plants for trypanocidal properties. Journal of Ethnopharmacol 77:19-24.
Agbedahunsi JM., Anao I, Adewunmi CO, Croft SL (2006). Trypanocidal properties of Terminalia ivorensis A. Chev (Combretaceae) Afr. J. Trad. Med 3(2):51-56.
Suffness M and Douros J (1979). Drugs of plant origin. Methods in Cancer Research 26:73–126.
Gilbert DWF , Kapche CD, Fozing JH and Abegaz BM (2009). Prenylated arylbenzofuran derivatives from Morus mesozygia with antioxidant activity. Phytochemistry 70:216-221
Mitsuo T, Shigemitsu N and Tadashi Ma. (1978). Structures of Moracins A and B, new phytoalexins from Diseased Mulberry. Tetrahedron Letters 9:797-798.
Emele FE, Izomoh MI and Alufohai E (1999). Microorganisms associated with wound infection in Ekpoma, Nigeria. West Afr J Med 18(2):97-100.
Malcom SA and Sofowora EA (1969). Antimicrobial activity of selected Nigerian folk remedies and their constituent plants. Lloydia 32:512-517.
Makhafola TJ, Samuel BB, Elgorashi EE and Eloff JN (2012). Ochnaflavone and Ochnaflavone 7-Omethyl Ether: two Antibacterial Biflavonoids from Ochna pretoriensis (Ochnaceae). Natural Products Communications 7(12):1601-1604.
Eloff JN (1998b). Which extracts should be used for the screening and isolation of antimicrobial compounds from plants ? Journal of Ethnopharmacol 60:1-8.
Hammerschmidt R and Dann EK (1999). The role of phytoalexins in plant protection. Novartis Found. Symp 223:175-187.
Mezencev R, Mojzis J, Pilatova M and Kutschy P (2003). Antiproliferative and cancer chemopreventive activity of phytoalexins: focus on
indole phytoalexins from crucifers. Neoplasma 50(4):239-45.