Anti-nociceptive and antipyretic activities of CoV-Pla 3 extract: relevance on symptomatic treatment of Covid-19 Disease

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Bukata B. Bukar
Kakjing D. Falang
Kennedy I. Amagon
Yusuf A. Agabi
Richard J. Kutshik
Ajima Ukpe
Yohanna L. Ishaya
Simji S. Gomerep
Stephen D. Davou
Ismaila Shittu
James G. Damen
Noel N. Wannang
 Jacob A. Kolawole

Abstract

Background: There is an urgent need to search for effective remedies against COVID-19 from the rich and extensive flora of Africa.


Objectives: The aim of this study was to evaluate the anti-nociceptive and anti-pyretic activities of the methanol extract of a herbal combination product, Cov-Pla3 in experimental animals.


Methods: Animal models of nociception and hyperthermia including acetic acid-induced contortion, hot-plateinduced nociception, egg albumin-induced paw edema and Brewer's yeast-induce hyperthermia were adopted.


Results: The results revealed that the Cov-Pla3 extract at all the doses used increased the reaction time in a nonsignificant manner (p > 0.05). However, the increase in reaction time at doses of the extract was significant compared to the baseline (p <0.05). The results also showed that Cov-Pla3 extract at the dose of 125 mg/kg at 90 minutes after administration had the highest anti-nociceptive effect of 49.73 % followed by 46.70 % at the dose of 500 mg/kg at same period. It was also shown that the extract significantly reduced acetic acid-contortions at a dose of 500 mg/kg (p < 0.05) with corresponding inhibition of 73 %. The extract at all the doses used significantly decreased paw edema compared to baseline after 180 minutes of administration (p < 0.05). The anti-pyretic test indicated that Cov-Pla3 significantly reduced temperature at 125 mg and 500 mg/kg after 4 hours of administration (p< 0.05). In conclusion, Cov-Pla3 possesses a combination of analgesic, anti-inflammatory and antipyretic activities.


Conclusion: This scientific evidence, in addition to its safety profile from the LD50 of above 5000 mg/kg, justifies its recommendation for clinical trial use in Covid-19 patients with severe symptoms.

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How to Cite
Bukar, B. B., Falang, K. D., Amagon, K. I., Agabi, Y. A., Kutshik, R. J., Ukpe, A., Ishaya, Y. L., Gomerep, S. S., Davou, S. D., Shittu, I., Damen, J. G., Wannang, N. N., & Kolawole, JacobA. (2023). Anti-nociceptive and antipyretic activities of CoV-Pla 3 extract: relevance on symptomatic treatment of Covid-19 Disease. West African Journal of Pharmacy, 31(1), 15-24. https://doi.org/10.60787/wapcp-31-1-186
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Articles
Author Biographies

Bukata B. Bukar, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

Department of Pharmacology and Toxicology, University of Jos, Jos

Kakjing D. Falang, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

Department of Pharmacology and Toxicology, University of Jos, Jos

Kennedy I. Amagon, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

Department of Pharmacology and Toxicology, University of Jos, Jos

Yusuf A. Agabi, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

Virology Unit, Department of Microbiology, University of Jos, Jos 

Richard J. Kutshik, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

Department of Biochemistry, University of Jos, Jos

Ajima Ukpe, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

Department of Pharmaceutical and Medicinal Chemistry, University of Jos, Jos

Yohanna L. Ishaya, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

Department of Biochemistry, University of Jos, Jos

Simji S. Gomerep, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

Infectious Disease Unit, Department of Medicine, University of Jos

Stephen D. Davou, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

Plateau State Ministry of Health, Jos

Ismaila Shittu, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

National Veterinary Research Institute (NVRI), Vom, Plateau state

James G. Damen, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

Department of Medical Laboratory Sciences, University of Jos, Jos 

Noel N. Wannang, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

Department of Pharmacology and Toxicology, University of Jos, Jos

 Jacob A. Kolawole, Plateau State Research Committee on Covid-19 and Other Infectious Diseases, Government House, Rayfield, Jos.

Department of Pharmaceutical and Medicinal Chemistry, University of Jos, Jos

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