Antinociceptive and anti-inflammatory properties of hydroethanolic seed extract of Monodora myristica (Annonaceae) in rodents
Main Article Content
Abstract
Background: Monodora myristica (Gaertn) Dunal (Annonaceae) is used in traditional medicine for cough, rheumatism, hemorrhoids, diabetes, anemia and headaches.
Objective: This study was carried out to investigate the antinociceptive and anti-inflammatory effects of the hydroethanolic seeds extract of Monodora myristca (HMM) in rodents.
Methods: HMM (50-200 mg/kg, p.o.) was administered 1 h before intraperitoneal or intraplantar injection of 0.6%V/v acetic acid or 1% formalin (20 µL), respectively, to evaluate the antinociceptive property. Acute and chronic anti-inflammatory effect was investigated using carrageenan-induced paw and xylene-induced ear oedema, and cotton-pellet induced granuloma tests, respectively.
Results: HMM (50-200 mg/kg) produced dose dependent and significant decrease in mean number of writhes in acetic acid-induced nociception and increased pain threshold to neurogenic and inflammatory pain with 48.59 and 34.2% inhibition, respectively, in the formalin-induced nociception assay. The HMMinduced antinociception was completely blocked by pre-treatment of mice with naloxone, pChlorophenyalanine (serotonin synthase inhibitor; 100 mg/kg, i.p.) and sulpiride (D2 receptor antagonist; 50mg/kg) whereas glibenclamide (KATP sensitive channels blocker; 10 mg/kg) failed to reverse the antinociceptive effect of the extract. In acute inflammatory model, HMM produced time course inhibition of carrageenan-induced paw oedema. In addition, pretreatment of mice with HMM inhibited xylene-induced ear oedema by 60% comparatively similar to the effect of dexamethasone (83.90%). Moreover, in the cottonpellet granuloma pouch, HMM (200 mg/kg) reduced granuloma formation by 52%.
Conclusion: The hydro-ethanolic seed extract of M. myristica possesses antinociceptive effect mediated through interaction with opioidergic, serotonergic and dopaminergic systems and an anti-inflammatory action through inhibition of inflammatory mediator's release. Finally, the study established the scientific basis for its use in the management of pain and inflammatory conditions in traditional medicine.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Chauhan SP, Sheth NR, Suhagia BN. (2015). Analgesic and Anti-inflammatory action of Opuntia elatior Mill fruits. Journal of Ayurveda and
Integrative Medicine, 6(2):75-81.
Burkill, H.M., 1994. The useful plants of West Tropical Africa. 2nd Edition. Volume 2, Families E–I. Royal Botanic Gardens, Kew, Richmond,
United Kingdom. 636 pp.
Bakarnga-Via I, Hzounda JB, Fokou PV, Tchokouaha LR, Gary-Bobo M, Gallud A, Garcia M, Walbadet L, Secka Y, Dongmo PM, Boyom FF, Menut C. (2014). Composition and cytotoxic activity of essential oils from Xylopia aethiopica (Dunal) A. Rich, Xylopia parviflora (A. Rich) Benth.) and Monodora myristica (Gaertn) growing in Chad and Cameroon. BMC Complementary and Alternative Medicine,14:125.
Moukette BM, Pieme CA, Njimou JR, Biapa CP, Marco B, Ngogang JY. (2015). In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a nontimber forest product used as spice: Monodora myristica. Biological Research, 48:15.
Tatsadjieu LN, Essia Ngang JJ, Ngassoum MB, Etoa FX. (2003). Antibacterial and antifungal activity of Xylopia ethiopica, Monodora myristica,
Zanthoxylum xanthoxyloides and Zanthoxylum leprieurii from Cameroon. Fitoterapia, 74(5):469-72.
Sofowora A (2006). Phytochemical screening. In: Medicinal plants and Traditional medicine in Africa. Second Edition, Spectrum Books Limited Ibadan, Nigeria.
Viturro C, Molina A, Schmeda-Hirschmann G. (1999). Free radical scavengers from Mutisia friesiana (Asteraceae) and Sanicula graveolens
(Apiaceae). Phytotherapy Research, 13:422-424.
The Organisation of Economic Co-operation and Development (OECD). (2001) The OECD Guideline for Testing of Chemical: 420 Acute Oral Toxicity, OECD, Paris,1-14.
Koster RM, Anderson M, De-Beer EJ. (1959) Acetic acid for analgesic screening. Federation Proceedings, 18:412–418.
Hunskaar S, Hole K. (1987). The formalin test in mice: Dissociation between inflammatory and non-inflammatory pain. Pain; 30:103–114.
Reisine T, Bell G.I. (1993). Molecular biology of opioid receptors. Trends in Neuroscience. 16:506 -10
Ishola IO, Awodele O, Olusayero AM, Ochieng CO. (2014). Mechanisms of Analgesic and AntiInflammatory Properties of Annona muricata Linn. (Annonaceae) Fruit Extract in Rodents. Journal of Medicinal Food 17(12):1375-82.
Bamgbose SOA, Noamesi BK. (1981). Studies on cryptolepine II: Inhibition of carrageenan-induced edema by cryptolepine. Planta Medica, 42:392-396.
Ishola IO, Akindele JA, Adeyemi OO (2011). Analgesic and Anti-inflammatory effect of methanolic root extract of Cnestis ferruginea Vahl
DC. Journal of Ethnopharmacology, 135:55–62.
Fabri RL, Garcia RA, Florêncio JR, Pinto NCC, de Oliveira LG, Aguiar JAK,Ribeiro A, Scio E. Antiinflammatory and antioxidative effects of the
methanolic extract of the aerial parts of Mitracarpus frigidus in established animal models. Journal of Pharmacy and Pharmacology, 2013;
:722–732.
Boyce-Rustay JM, Honore P, Jarvis MF. (2010). Animal models of acute and chronic inflammatory and nociceptive pain. Methods and Molecular Biology, 617: 41-55.
Okuse K. (2007). Pain signalling pathways: from cytokines to ion channels. International Journal of Biochemistry and Cell Biology, 39(3): 490-6.
Ishola IO, Ashorobi RB, Adeoluwa O. (2012). Evaluation of the antinociceptive activities of the aqueous root extract of Alchornea cordifolia
( Schumach and Thonn) Müll. Arg. (Euphorbiaceace). International Journal of Applied Research in Natural Products 5 (3): 37-42.
Dickensen A.H. (1994). Where and how do opioids act? Proceedings of the 7th World Congress on Pain. In: Gebhart G.F., Hammond D.L., Jensen T.S., editors. Progress in pain research and management. 2: 525 – 52.
Wood PB (2008). Role of central dopamine in pain and analgesia. Expert Rev Neurother. 8:781-797.
Altier N, Stewart J (1999). The role of dopamine in the nucleus accumbens in analgesia. Life Science. 65:2269-2287.
Taylor BK, Joshi C, Uppal H (2003). Stimulation of dopamine D2 receptors in the nucleus accumbens inhibits inflammatory pain. Brain Research. 987:135-143.
Linley JE, Rose K, Ooi L, Gamper N (2010). Understanding inflammatory pain: ion channels contributing to acute and chronic nociception.
Pflugers Archives. 459, 657–669 (2010). doi:10.1007/s00424-010-0784-6
Ocana M, Del Pozo E, Barrios M, Robles LI, Baeyens JM (1990). An ATP-dependent potassium channel blocker antagonizes morphine analgesia. European Journal of Pharmacology. 186, 377–378. doi:10.1016/0014-2999(90)90466-J
Robles LI, Barrios M, Del Pozo E, Dordal A, Baeyens JM (1996). Effects of K+ channel blockers and openers on antinociception induced by agonists of 5-HT1A receptors. European Journal of Pharmacology. 295, 181–188. doi:10.1016/0014- 2999(95)00643-5
Rodrigues AR., Duarte., ID. (2000). The peripheral antinociceptive effect induced by morphine is associated with ATP-sensitive K(+) channels. British Journal of Pharmacology. 129: 110–114. doi:10.1038/sj.bjp.0703038
Zaninir JC, Medeiros YS, Cruz AB, Yunes RRA, Calixto JB. (1992). Action of compounds from Mandevilla velutina on croton oil induced ear
edema in mice; A comparative study with steroid and non-steroidal anti-inflammatory drugs. Phytotherapy Research 6: 1-5
Gamache DA, Povlishock JT, Ellis EF (1986). Carrageenan induced brain inflammation: characterization of the model. Journal of
Neurosurgery. 65; 5: 679 - 685
Kou, Jm, Ma, Rm, Zhu, Dm and Yan, Y. 2003. Bloodactivating and anti-inflammatory actions of Polygala fallax. Zhong Yao Cai, 26: 268-71.
Ahmad, R., Shaari, K., Lagis, N. H., Hamzah, A. S., Ismail, N. H., & Kitayima, M. (2005). Anthraquinones from Hedyotis capitellata. Phytochemistry, 66: 114-1147. http://dx.doi.org/10.1016/j.phytochem. 2005.02.023 Bhaskar R, Rajeswari V and Sathish KT (2007). In
vitro antioxidant studies in leaves of Annona species. Indian Journal of Experimental Biology 4: 480−485.