Antioxidant activity, total phenolic and flavonoid contents of Anthocleista djalonensis root extracts

Main Article Content

Patrick O. Igbinaduwa
 Benjamin U. Okeke

Abstract

Background: Anthocleista djalonensis (Chev.), a plant native to tropical West Africa, is used ethnomedicinally in the management of several diseases.


Objectives: This study seeks to determine the free radical scavenging ability of the methanol, ethyl acetate and aqueous root extracts of A. djalonensis on 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide radicals and to determine the total phenolic and flavonoid contents of the extracts.


Method: The antioxidant effect of A. djalonensis root extracts was compared with that of ascorbic acid using DPPH (2, 2- diphenyl-2- picrylhydrazyl) assay and hydrogen peroxide scavenging assay methods. Percentage antioxidant activity (AA %), IC50, total phenolic content and total flavonoid of extracts were determined.


Result: The total phenolic content of the extracts ranges from 132.73 ± 20.25 to 207.93 ± 11.7 mgGAE/g of dry plant extract while the total flavonoid content of the extracts ranges from 78.2 ± 0.12 to 100.6 ± 2.08 mg QE/g dry plant extract. The extracts showed a good but lower DPPH radical scavenging activity compared to ascorbic acid. The methanol extract showed a maximum percentage radical scavenging of 94.76 ± 0.05% (IC50 = 148.54 µ/ml) on DPPH radicals at concentration of 1000 µg/ml among the three extracts. The extracts showed a non-significant (P ˃ 0.05) radical scavenging activity on hydrogen peroxide compared to ascorbic acid (74.14 ± 0.05 %). The methanol extract have the highest hydrogen peroxide scavenging activity (79.24 ± 0.07 %).


Conclusion: This result indicate that the root extracts of Anthocleista djalonensis possesses antioxidant phytochemicals and can be a good source of antioxidants to ameliorate conditions that are caused by oxidative effects of free radicals.

Downloads

Download data is not yet available.

Article Details

How to Cite
Igbinaduwa, P. O., & Okeke, BenjaminU. (2023). Antioxidant activity, total phenolic and flavonoid contents of Anthocleista djalonensis root extracts. West African Journal of Pharmacy, 30(1), 134-142. https://doi.org/10.60787/wapcp-30-1-181
Section
Articles

References

Molyneux P (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin

Journal of Science and Technology; 26:211-219.

Valko M, Leibfritz D, Moncol J, Cronin M, Mazur M (2007). Free radicals and antioxidants in normal physiological functions and human

disease. International Journal Biochem Cell Biol; 39 (1): 44-84.

Harborne JB, Baxter H, Moss GP (1999). Phytochemical Dictionary: Handbook of Bioactive Compounds from Plants, Taylor & Francis, London, UK, 2nd edition,.

Enrique C, Davies KJA (2000). Mitochondrial free radical generation, oxidative stress, and aging. Free Radical Biology and Medicine; 29(3):222-230.

Catala A (2009). Lipid peroxidation of membrane phospholipids generates hydroxyalkanals and oxidized Phospholipids active in physiological and/or pathological conditions. Chem. Phys. Lipids; 157 (1):1-11.

Antolovic M, Prenzler PD, Patsalides E, McDonald S, Robards K (2002). Methods for testing antioxidant activity. Analyst, 127: 183–198.

Mascio PD, Murphy ME, Sies H. (1991). Antioxidant defense systems: the role of carotenoids, tocopherols, and thiols. The American Journal of Clinical Nutrition; 53: 194 - 200.

Cruz M, Franco D, Dominguez JM, Senerio J, Dominguez H, Nunez, MJ (2001). “Natural antioxidants form residual sources,” Food

Chemistry, 72(2):145–171,.

Burkill HM (1985). The Useful Plants of West Tropical Africa. Royal Botanical Gardens, Vol. 3.

Erhabor JO, Idu M, Udo FO (2013). Ethnomedicinal Survey of Medicinal Plants Used in the Treatment of Male Infertilty among the IFA Nkari People of Ini Local Government Area of Akwa Ibom State, Nigeria. Research Journal of Recent Medicines; 2: 5-11.

Anyanwu GO, Nisar R, Onyeneke CE, Rauf K (2015). Medicinal plants of the genus Anthocleista - A review of their ethnobotany, phytochemistry and pharmacology. Journal of Ethnopharmacology; 175: 648 – 667.

Sofowora A (1993). Medicinal Plants and Traditional Medicinal in Africa.2nd Ed. Sunshine House, Ibadan, Nigeria: Spectrum

Books Ltd; Screening Plants for Bioactive Agents; pp. 134–156.

Trease GE, Evans WC (2002). Pharmacognosy. 15th Ed. London: Saunders Publishers; 42–44. 221–229, 246–249, 304–306, 331–332,

–393.

Mensor LL, Menezes FS, Leitao GG, Reis AS, Dos Santos C, Coube CS, Leitao SG (2001). Screening of Brazilian plant extracts for

antioxidant activity by the use of DPPH free radical method. Phytother Research; 15: 127–130.

Ruch RJ, Cheng SJ, Klaunig JE (1989). Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins

isolated from Chinese greentea. Carcinogenesis, 10, pp. 1003-1008.

Singleton VL, Orthofer R, Lamuela-Raventos RM (1999). Analysis of total phenols and other oxidation subtrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymology 299:152-178.

Mervat MM, Far E, Hanan A, Taie A (2009). Antioxidant activities, total anthrocynins, phenolics and flavonoid contents of some sweet potato genotypes under stress of different concentration of sucrose and sorbitol. Australian Journal of Basic Applied Science, 3(4):3609-3616.

Halvorsen BL, Holte K, Myhrstad MCW, Barikmo I, Hvattum, E, (2002). A systematic screening of total antioxidants in dietary plants. J Nutr 132: 461-471.

Rahal A, Ahmad AH, Kumar A (2013). “Clinical drug interactions: a holistic view,” Pakistan Journal of Biological Sciences, 16(16): 751–758.

Mosquera OM, Correa YM, Buitrago DC, Nio J (2007). Anti-oxidant activity of twenty five plants from Colombian biodiversity. Memorias do instituto Oswando Cruz, 102: 631-634.

Patel RP, Cornwell T, Darley-Usmar VM (1999). "The biochemistry of nitric oxide and peroxynitrite: implications for mitochondrial

function". In Packer L , Cadenas E. Understanding the process of aging: the roles of mitochondria, free radicals, and antioxidants. New York, NY: Marcel Dekker. pp. 39–56.

Turrens JF (2003). "Mitochondrial formation of reactive oxygen species". The Journal of Physiology. 552 (2): 335–4.

Ammar A, Naoufal L, Azam B, Dennis GW, David AL (2017). Phytochemicals: Extraction, Isolation, and Identification of Bioactive

Compounds from Plant Extracts, Journal of Plants; 6 (4): 42.

Similar Articles

You may also start an advanced similarity search for this article.